Hi,
According to your description, I assume that it may be caused by your IIS 5 configurations.
1. It works on IIS 6, indicates the application is OK.
2. It works with VS web server, indicates the local machine.config setting is OK.
As a result, the configuration in IIS 5 may be wrong.
Please check this carefully. Or try to create another application in IIS 5 with default settings.
Hope this helps.
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